Review



mightyscript plus first strand cdna synthesis master mix  (Sangon Biotech)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Sangon Biotech mightyscript plus first strand cdna synthesis master mix
    Mightyscript Plus First Strand Cdna Synthesis Master Mix, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mightyscript+plus+first+strand+cdna+synthesis+master+mix/cdna+first+kit+mirna+strand+synthesis/pm42297343-57-21-34
    Average 86 stars, based on 1 article reviews
    mightyscript plus first strand cdna synthesis master mix - by Bioz Stars, 2026-09
    86/100 stars

    Images

    Related Articles

    Synthesized:

    Article Title: Biocontrol Mechanisms of a Chinese Heterorhabditis indica Strain Against Tuta absoluta : Virulence Assay and Time-Course Transcriptomics of Host Immune Responses
    Article Snippet: .. First-strand cDNA was synthesized from 1 μg of total RNA per sample using a commercial MightyScript Plus First Strand cDNA Synthesis Master Mix (Sangon Biotech, Shanghai, China, Cat# B639252), following the manufacturer’s protocol. .. The resulting cDNA was then diluted ten-fold with nuclease-free water and used as the template for subsequent qPCR reactions. qPCR was performed using the SGExcel Universal SYBR qPCR Mix (Sangon Biotech, Shanghai, China, Cat# B532955) on a qTower 3 real-time PCR system (Analytik Jena, Jena, Thuringia, Germany).

    Article Title: Biocontrol Mechanisms of a Chinese Heterorhabditis indica Strain Against Tuta absoluta : Virulence Assay and Time-Course Transcriptomics of Host Immune Responses.
    Article Snippet: .. First-strand cDNA was synthesized from 1 μg of total RNA per sample using a commercial MightyScript Plus First Strand cDNA Synthesis Master Mix (Sangon Biotech, Shanghai, China, Cat# B639252), following the manufacturer’s protocol. .. The resulting cDNA was then diluted ten-fold with nuclease-free water and used as the template for subsequent qPCR reactions. qPCR was performed using the SGExcel Universal SYBR qPCR Mix (Sangon Biotech, Shanghai, China, Cat# B532955) on a qTower3 real-time PCR system (Analytik Jena, Jena, Thuringia, Germany).

    Article Title: The genome of an aneuploid cultivar facilitates elucidating the genetic bases of trait polymorphisms in Phalaenopsis orchids
    Article Snippet: To confirm the silencing of target genes in plants showing phenotypic changes, total RNA was extracted from the corresponding floral tissues using the FastPure Universal Plant Total RNA Isolation Kit (Vazyme, Nanjing, China). .. First-strand cDNA was synthesized using MightyScript Plus First Strand cDNA Synthesis Master Mix (gDNA digester) (Sangon Biotech, Shanghai, China). .. Quantitative reverse-transcription PCR (qRT-PCR) was performed using SupRealQ Ultra Hunter SYBR qPCR Master Mix (U+) (Vazyme).

    Article Title: Schisandrol A induces cardiac developmental toxicity in zebrafish (Danio rerio) via disruption of calcium homeostasis, oxidative stress, and apoptosis.
    Article Snippet: Schisandrol A (SA) is a major lignan component of Schisandra chinensis with anti-inflammatory and antioxidant activities, yet its developmental and cardiac safety profile remains unclear.. In this study, a zebrafish (Danio rerio) embryo model was used to evaluate the toxicity of SA and its underlying mechanisms following exposure to different concentrations of SA (ranging from 0 to 160 μg/mL, with safe concentrations of 10, 20, and 40 μg/mL for mechanistic studies from 6 to 96 h post-fertilization (hpf).. SA induced a dose-dependent increase in mortality, with an LC50 of 62.5 μg/mL at 96 hpf.

    Article Title: Zebrafish (Danio rerio)-based assessment of Rhododendron dauricum developmental and cardiotoxicity: Calcium overload, oxidative stress, and apoptosis mediated by rhododendrol.
    Article Snippet: Ethnopharmacological relevance: Rhododendron dauricum L. is used in traditional medicine for respiratory disorders, but its developmental and cardiac safety remains insufficiently defined.. Aim of the study: To characterize developmental/cardiac phenotypes induced by Rhododendron dauricum hotwater extract (RDWE), identify toxicity-enriched fractions and candidate constituents, and assess whether selective fraction removal reduces toxicity-related effects.. Materials and methods: Zebrafish embryos/larvae were exposed to RDWE, four membrane fractions, and candidate monomers.

    cDNA Synthesis:

    Article Title: Biocontrol Mechanisms of a Chinese Heterorhabditis indica Strain Against Tuta absoluta : Virulence Assay and Time-Course Transcriptomics of Host Immune Responses
    Article Snippet: .. First-strand cDNA was synthesized from 1 μg of total RNA per sample using a commercial MightyScript Plus First Strand cDNA Synthesis Master Mix (Sangon Biotech, Shanghai, China, Cat# B639252), following the manufacturer’s protocol. .. The resulting cDNA was then diluted ten-fold with nuclease-free water and used as the template for subsequent qPCR reactions. qPCR was performed using the SGExcel Universal SYBR qPCR Mix (Sangon Biotech, Shanghai, China, Cat# B532955) on a qTower 3 real-time PCR system (Analytik Jena, Jena, Thuringia, Germany).

    Article Title: Therapeutic effect of eravacycline against carbapenem-resistant hypervirulent Klebsiella pneumoniae in mouse models
    Article Snippet: Total RNA was extracted from the lung tissue using the RNA Easy Fast Tissue/Cell kit (Tiangen, Beijing, China). .. The extracted RNA was reverse transcribed into cDNA using MightyScript Plus First Strand cDNA Synthesis Master Mix (Sangon Biotech, Shanghai, China), and then reverse transcription-quantitative PCR (RT-qPCR) was performed. ..

    Article Title: Biocontrol Mechanisms of a Chinese Heterorhabditis indica Strain Against Tuta absoluta : Virulence Assay and Time-Course Transcriptomics of Host Immune Responses.
    Article Snippet: .. First-strand cDNA was synthesized from 1 μg of total RNA per sample using a commercial MightyScript Plus First Strand cDNA Synthesis Master Mix (Sangon Biotech, Shanghai, China, Cat# B639252), following the manufacturer’s protocol. .. The resulting cDNA was then diluted ten-fold with nuclease-free water and used as the template for subsequent qPCR reactions. qPCR was performed using the SGExcel Universal SYBR qPCR Mix (Sangon Biotech, Shanghai, China, Cat# B532955) on a qTower3 real-time PCR system (Analytik Jena, Jena, Thuringia, Germany).

    Article Title: The genome of an aneuploid cultivar facilitates elucidating the genetic bases of trait polymorphisms in Phalaenopsis orchids
    Article Snippet: To confirm the silencing of target genes in plants showing phenotypic changes, total RNA was extracted from the corresponding floral tissues using the FastPure Universal Plant Total RNA Isolation Kit (Vazyme, Nanjing, China). .. First-strand cDNA was synthesized using MightyScript Plus First Strand cDNA Synthesis Master Mix (gDNA digester) (Sangon Biotech, Shanghai, China). .. Quantitative reverse-transcription PCR (qRT-PCR) was performed using SupRealQ Ultra Hunter SYBR qPCR Master Mix (U+) (Vazyme).

    Article Title: Effects of curcumin supplementation in high-fat diet on growth performance, antioxidant capacity and lipid metabolism of spotted sea bass (Lateolabrax maculatus).
    Article Snippet: This study aimed to investigate the effects of curcumin supplementation in high-fat diets on growth performance, antioxidant capacity and lipid metabolism in spotted sea bass (Lateolabrax maculatus).. Six experimental diets were formulated: a normal-fat diet (NF), a high-fat diet (HF), and four experimental diets derived from the HF supplemented with 0.02%, 0.04%, 0.08%, and 0.16% curcumin.. A total of 450 healthy spotted sea bass, with an initial body weight of (2.00 ± 0.16) g, were selected and randomly allocated into 6 groups (3 replicates per group, 25 fish per replicate).

    Article Title: Schisandrol A induces cardiac developmental toxicity in zebrafish (Danio rerio) via disruption of calcium homeostasis, oxidative stress, and apoptosis.
    Article Snippet: Schisandrol A (SA) is a major lignan component of Schisandra chinensis with anti-inflammatory and antioxidant activities, yet its developmental and cardiac safety profile remains unclear.. In this study, a zebrafish (Danio rerio) embryo model was used to evaluate the toxicity of SA and its underlying mechanisms following exposure to different concentrations of SA (ranging from 0 to 160 μg/mL, with safe concentrations of 10, 20, and 40 μg/mL for mechanistic studies from 6 to 96 h post-fertilization (hpf).. SA induced a dose-dependent increase in mortality, with an LC50 of 62.5 μg/mL at 96 hpf.

    Article Title: Therapeutic effect of eravacycline against carbapenem-resistant hypervirulent Klebsiella pneumoniae in mouse models.
    Article Snippet: Total RNA was extracted from the lung tissue using the RNA Easy Fast Tissue/Cell kit (Tiangen, Beijing, China). .. The extracted RNA was reverse transcribed into cDNA using MightyScript Plus First Strand cDNA Synthesis Master Mix (Sangon Biotech, Shanghai, China), and then reverse transcription-quantitative PCR (RT-qPCR) was performed. ..

    Article Title: Zebrafish (Danio rerio)-based assessment of Rhododendron dauricum developmental and cardiotoxicity: Calcium overload, oxidative stress, and apoptosis mediated by rhododendrol.
    Article Snippet: Ethnopharmacological relevance: Rhododendron dauricum L. is used in traditional medicine for respiratory disorders, but its developmental and cardiac safety remains insufficiently defined.. Aim of the study: To characterize developmental/cardiac phenotypes induced by Rhododendron dauricum hotwater extract (RDWE), identify toxicity-enriched fractions and candidate constituents, and assess whether selective fraction removal reduces toxicity-related effects.. Materials and methods: Zebrafish embryos/larvae were exposed to RDWE, four membrane fractions, and candidate monomers.

    Reverse Transcription:

    Article Title: Therapeutic effect of eravacycline against carbapenem-resistant hypervirulent Klebsiella pneumoniae in mouse models
    Article Snippet: Total RNA was extracted from the lung tissue using the RNA Easy Fast Tissue/Cell kit (Tiangen, Beijing, China). .. The extracted RNA was reverse transcribed into cDNA using MightyScript Plus First Strand cDNA Synthesis Master Mix (Sangon Biotech, Shanghai, China), and then reverse transcription-quantitative PCR (RT-qPCR) was performed. ..

    Article Title: Effects of curcumin supplementation in high-fat diet on growth performance, antioxidant capacity and lipid metabolism of spotted sea bass (Lateolabrax maculatus).
    Article Snippet: This study aimed to investigate the effects of curcumin supplementation in high-fat diets on growth performance, antioxidant capacity and lipid metabolism in spotted sea bass (Lateolabrax maculatus).. Six experimental diets were formulated: a normal-fat diet (NF), a high-fat diet (HF), and four experimental diets derived from the HF supplemented with 0.02%, 0.04%, 0.08%, and 0.16% curcumin.. A total of 450 healthy spotted sea bass, with an initial body weight of (2.00 ± 0.16) g, were selected and randomly allocated into 6 groups (3 replicates per group, 25 fish per replicate).

    Article Title: Therapeutic effect of eravacycline against carbapenem-resistant hypervirulent Klebsiella pneumoniae in mouse models.
    Article Snippet: Total RNA was extracted from the lung tissue using the RNA Easy Fast Tissue/Cell kit (Tiangen, Beijing, China). .. The extracted RNA was reverse transcribed into cDNA using MightyScript Plus First Strand cDNA Synthesis Master Mix (Sangon Biotech, Shanghai, China), and then reverse transcription-quantitative PCR (RT-qPCR) was performed. ..

    Polymerase Chain Reaction:

    Article Title: Therapeutic effect of eravacycline against carbapenem-resistant hypervirulent Klebsiella pneumoniae in mouse models
    Article Snippet: Total RNA was extracted from the lung tissue using the RNA Easy Fast Tissue/Cell kit (Tiangen, Beijing, China). .. The extracted RNA was reverse transcribed into cDNA using MightyScript Plus First Strand cDNA Synthesis Master Mix (Sangon Biotech, Shanghai, China), and then reverse transcription-quantitative PCR (RT-qPCR) was performed. ..

    Article Title: Therapeutic effect of eravacycline against carbapenem-resistant hypervirulent Klebsiella pneumoniae in mouse models.
    Article Snippet: Total RNA was extracted from the lung tissue using the RNA Easy Fast Tissue/Cell kit (Tiangen, Beijing, China). .. The extracted RNA was reverse transcribed into cDNA using MightyScript Plus First Strand cDNA Synthesis Master Mix (Sangon Biotech, Shanghai, China), and then reverse transcription-quantitative PCR (RT-qPCR) was performed. ..

    Purification:

    Article Title: Schisandrol A induces cardiac developmental toxicity in zebrafish (Danio rerio) via disruption of calcium homeostasis, oxidative stress, and apoptosis.
    Article Snippet: Schisandrol A (SA) is a major lignan component of Schisandra chinensis with anti-inflammatory and antioxidant activities, yet its developmental and cardiac safety profile remains unclear.. In this study, a zebrafish (Danio rerio) embryo model was used to evaluate the toxicity of SA and its underlying mechanisms following exposure to different concentrations of SA (ranging from 0 to 160 μg/mL, with safe concentrations of 10, 20, and 40 μg/mL for mechanistic studies from 6 to 96 h post-fertilization (hpf).. SA induced a dose-dependent increase in mortality, with an LC50 of 62.5 μg/mL at 96 hpf.

    Article Title: Zebrafish (Danio rerio)-based assessment of Rhododendron dauricum developmental and cardiotoxicity: Calcium overload, oxidative stress, and apoptosis mediated by rhododendrol.
    Article Snippet: Ethnopharmacological relevance: Rhododendron dauricum L. is used in traditional medicine for respiratory disorders, but its developmental and cardiac safety remains insufficiently defined.. Aim of the study: To characterize developmental/cardiac phenotypes induced by Rhododendron dauricum hotwater extract (RDWE), identify toxicity-enriched fractions and candidate constituents, and assess whether selective fraction removal reduces toxicity-related effects.. Materials and methods: Zebrafish embryos/larvae were exposed to RDWE, four membrane fractions, and candidate monomers.



    Similar Products

    86
    Sangon Biotech mightyscript plus first strand cdna synthesis master mix
    Mightyscript Plus First Strand Cdna Synthesis Master Mix, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mightyscript+plus+first+strand+cdna+synthesis+master+mix/cdna+first+kit+mirna+strand+synthesis/pm42297343-57-21-34
    Average 86 stars, based on 1 article reviews
    mightyscript plus first strand cdna synthesis master mix - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Sangon Biotech mightyscript plus first strand cdna synthesis master mix kit
    Mightyscript Plus First Strand Cdna Synthesis Master Mix Kit, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mightyscript+plus+first+strand+cdna+synthesis+master+mix/cdna+first+kit+mirna+strand+synthesis/pm41871234-65-12-21
    Average 86 stars, based on 1 article reviews
    mightyscript plus first strand cdna synthesis master mix kit - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    Image Search Results